Coding

Part:BBa_K2638211:Design

Designed by: Jakob Zubek   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


Tn501MerA (S558H, S559H)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1311
    Illegal AgeI site found at 1081
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1387


Design Notes

The replacement of the both cysteines with histidine should lead to a higher affinity towards copper ions.



Source

Gene synthesis by IDT (originally found in: Pseudomonas aeruginosa)

References